| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Paez, Isabella S (17)
|
MRBGNW |
25.49 |
25.52 |
0.03 |
| 2 |
Zorgman, Sunell (15)
|
MRBGNW |
24.93 |
25.99 |
1.06 |
| 3 |
Alexander, Sinceraty D (17)
|
MRBGNW |
24.48 |
26.19 |
1.71 |
| 4 |
Slive, Ellie R (15)
|
MRBGNW |
25.35 |
26.26 |
0.91 |
| 5 |
McIntyre, Savannah Y (16)
|
MRBGNW |
25.64 |
26.29 |
0.65 |
| 5 |
Raath, Ella (17)
|
MRBGNW |
25.77 |
26.29 |
0.52 |
| 7 |
Tan, Marisol N (15)
|
MRBGNW |
25.46 |
26.31 |
0.85 |
| 8 |
Wohl, Katharine A (18)
|
MRBGNW |
25.64 |
26.48 |
0.84 |
| 9 |
Sun, Julia (15)
|
MRBGNW |
26.84 |
26.79 |
-0.05 |
| 10 |
Patterson, Clare M (16)
|
MRBGNW |
26.96 |
26.90 |
-0.06 |
| 11 |
Tello, Lizette (16)
|
MRBGNW |
28.02 |
28.17 |
0.15 |
| 12 |
Hammond, Amelia C (15)
|
MRBGNW |
27.92 |
28.30 |
0.38 |
| 13 |
Tsang, Jessica (16)
|
MRBGNW |
27.51 |
28.39 |
0.88 |
| 14 |
Smith, Alexandria K (17)
|
MRBGNW |
29.31 |
28.68 |
-0.63 |